1. A Stabilized Stannous Fluoride Dentifrice: In Vitro Studies of Anticaries Potential

A 24-Hour Dental Plaque Prevention Study with a Stannous Fluoride Dentifrice

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Reference: Pfarrer AM, McQueen CM, Lawless MA, Rapozo-Hilo M, Featherstone JDB. Compend Contin Educ Dent. 2005;26(Suppl1):41-46.

CONCLUSION

In vitro studies demonstrated the anticaries potential of the stabilized stannous fluoride dentifrice.

OBJECTIVE

To examine the anticaries potential of a stabilized stannous fluoride dentifrice with sodium hexametaphosphate (for cosmetic benefits).

MATERIALS AND METHODS

In vitro anti-caries profile methods were:

  • Fluoride uptake into demineralized enamel: single-treatment, mechanism-of-action study.
  • Remineralization/inhibition of demineralization: multiple-treatment study under lesion progression pH-cycling conditions. Dentifrices compared in the respective profile methods were:
  •  

  • Fluoride uptake
  • - Stabilized stannous fluoride with sodium hexametaphosphate (1,100 pmm fluoride as stannous fluoride, sodium hexametaphosphate, and silica)

    - United States Pharmacopeia (USP) Reference Standard (1,100 pmm fluoride as stannous fluoride and silica)

    - Dose-response control USP Reference Standard (diluted to 250 ppm fluoride as stannous fluoride and silica)

    - Placebo negative control (<1ppm fluoride and silica)

     

  • Remineralization/inhibition of demineralization
  • - Stabilized stannous fluoride with sodium hexametaphosphate

    - Sodium fluoride with sodium hexametaphosphate (1,100 pmm fluoride as sodium fluoride, sodium hexametaphosphate, and silica)

    - Stannous fluoride USP Reference Standard (1,100 pmm fluoride as stannous fluoride and silica)

    - Sodium fluoride USP Reference Standard (1,100 ppm fluoride as sodium fluoride and silica)

    - Dose-response sodium fluoride control

    - Placebo negative control (<1ppm fluoride)

     

  • Fluoride uptake
  • Human enamel samples from extracted teeth – 3 mm diameter cores – were decalcified for 24 hours to produce early caries lesions 20-30 μm deep. Samples were taken from the cores by the microdrill biopsy technique. Samples were measured for fluoride levels pre-dentifrice treatment. Groups of specimens were treated with dentifrice/saliva slurries. Samples were taken to determine post-treatment fluoride levels. The difference between pre and post levels determined fluoride uptake.

  • Remineralization/inhibition of demineralization
  • Caries-free human molar or premolar crowns were each treated to produce a 3 x 2 mm window on one surface as the entry point for demineralization. 24-hour test cycles

    - 6 hours demineralization, 1 minute dentifrice treatment, 16 hours remineralization, 1 minute treatment – were repeated for 14 days. Cycles were designed to model normal demineralization and remineralization. The resulting lesions were measured for progression into the enamel, and mineral loss from each lesion calculated.

RESULTS

  • Fluoride uptake
  • There was no statistically significant difference between the stannous fluoride with sodium hexametaphosphate toothpaste and the stannous fluoride USP Reference Standard toothpaste.

  • Remineralization/inhibition of demineralization
  • The stannous fluoride with sodium hexametaphosphate toothpaste was at least as good as the clinically proven stannous fluoride and sodium fluoride USP Reference Standard toothpastes.

Stannous fluoride and sodium fluoride USP Reference Standard toothpastes
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