During the course of diagnostic and therapeutic services in oral healthcare settings, instruments and other devices come in contact with the patient’s skin, mucous membranes, sterile tissues, and body fluids. Failure to properly sterilize, or minimally disinfect, patient-care items carries the risk of healthcare-associated transmission of infectious diseases. This course presents evidence-based information for the preferred methods of transporting, cleaning, sterilizing and disinfecting patient-care items in oral healthcare settings.