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Aerosols in the Dental Office: Best Practices for Patient and Practitioner Safety

Course Number: 619

Characteristics of SARS-CoV-2

Coronaviruses (Coronaviridae, of the order Nidovirales) are large, single stranded RNA viruses.49,50 Currently, there are four known genera of coronaviruses: α-CoV, β-CoV, γ-CoV, and δ-CoV.51,52 Coronaviruses have been identified as the causative agents of diseases in humans and other vertebrates. SARS-CoV-2 belongs to the β-COV family, which along with α-CoV viruses, are known to infect mammals and humans.49,53,54 SARS-CoV-2 possesses an ultrastructure typical of other coronaviruses, namely a membrane envelope with multiple “spike glycoprotein” (S-protein) extensions (Figure 1).55 The viral capsule has also been found to express other polyproteins, nucleoproteins, and membrane proteins, including specifically RNA polymerase, 3-chymotrypsin-like protease, papain-like protease, helicase, glycoprotein, and accessory proteins.7,55,56 The S-proteins from coronaviruses binds to receptors on host cells to facilitate viral entry into the target cells. For SARS-CoV-2 the target receptor is the human angiotensin-converting enzyme 2 receptor (ACE2).57-60 Affinity for the ACE2 receptor is postulated to explain increased viral loads seen in older individuals, since ACE2 expression increases with age.61

Illustration diagramming the ultrastructure of the SAR-CoV-s virus.

Figure 1. Diagram of the ultrastructure of the SARS-CoV-2 virus.91

It is well-established that RNA viruses have higher rates of mutation than DNA-viruses.62 On a per-site level, DNA viruses typically have mutation rates on the order of 10−8 to 10−6 substitutions per nucleotide site per cell infection (s/n/c). RNA viruses have mutation rates that range between 10−6 and 10−4 s/n/c. Given this rapid mutation rate, RNA viruses with high rates of pathogenicity are able generally to develop and propagate more frequently in the environment.62 However, current data suggests that SARS-CoV-2 may have a lower mutation rate than influenza, which may be advantageous during the development of vaccines and target medications.63 Additionally, the reproduction number (R0) of SARS-CoV-2 has been estimated to be between 1.5-6.49 with a median value of 2.78.64 This R0 is greater than that of SARS-CoV-1 (median R0=1.3)65 and H1N1 influenza (median R0=1.46),66" but less than that of measles (median R0=16.1).67

SARS-CoV-2 shares numerous similarities with SARS-CoV-1 [the causative agent in the severe acute respiratory syndrome (SARS) outbreak in 2002-2003], but there are some significant differences. Both originated in China and both demonstrate stability in the environment. In an in vitro study, SARS-CoV-2 was detectable in aerosols for up to three hours, up to four hours on copper, up to 24 hours on cardboard and up to two to three days on plastic and stainless steel.68 However, while SARS-CoV-1 was eradicated by intensive contact tracing and case isolation measures and no cases have been detected since 2004.69 SARS-CoV-2 has proven to be significantly more difficult to eradicate. Emerging evidence suggests that asymptomatic people infected with SARS-CoV-2 may be transmitting the virus prior to the onset of symptoms.70 The occurrence of asymptomatic transmission decreases the effectiveness of disease control measures that were effective against SARS-CoV-1.70 In contrast to SARS-CoV-1, most secondary cases of virus transmission of SARS-CoV-2 appear to be occurring in community settings.70

Both of viruses demonstrate binding affinity to the ACE2 receptor to enter host cells. However, the S-proteins from SARS-CoV-2 are less stable than those of SARS-CoV-1 and polyclonal anti-SARS S1 antibodies that inhibit entry of SARS-CoV-1, are not effective against SARS-CoV-2 pseudovirions.71 Further studies using recovered SARS and COVID-19 patients’ sera show limited cross-neutralization, suggesting that recovery from one infection might not protect against the other.71